GIF inhibits Th effector generation by acting on antigen-presenting B cells.

摘要: Glycosylation-inhibiting factor (GIF) is a 13-kDa cytokine secreted from T cells. Administration of bioactive recombinant GIF inhibits IgG1 and IgE Ab responses in vivo. Treatment B cells with the reduces secretion induced by LPS IL-4. To examine effect on cognate T-B interaction, was added to low-density MD4 transgenic (Tg) mice, which express cell receptor specific for hen egg lysozyme (HEL). The were subsequently pulsed HEL-OVA conjugate cultured OVA-specific naive CD4 DO11.10 Tg mice. Ag-presenting reduced expansion IL-2 rendered them hyporesponsive antigenic restimulation, resulting 50--95% reduction IL-4 IFN-gamma upon restimulation Ag. dramatically inhibited Th effector generation when it before pulsing HEL-OVA, whereas showed little no after more effective mice than they OVA. This did not affect or irradiated splenocytes OVA(323--339) peptide stimulated Confocal microscopy revealed that internalization HEL Therefore, may regulate early steps Ag presentation involving receptors diminish