Mechanisms for targeting of the Saccharomyces cerevisiae GPI-anchored cell wall protein Crh2p to polarised growth sites.

摘要: The cell wall is an essential structure that preserves the osmotic integrity of fungal cells and determines cellular morphology during developmental programs. high number different components demands a variety processes to deliver precursors synthetic proteins the proper location at right time for development modification. Here, the specificity mechanisms regulate temporal spatial localisation sites polarised growth in Saccharomyces cerevisiae investigated. For this purpose, the localisation Crh2p, a glycosylphosphatidylinositol (GPI)-anchored mannoprotein we have recently described as involved in wall construction localised sites, was followed using a Crh2p-GFP fusion protein. Crh2p distribution studied several genetic backgrounds affected steps polarity establishment machinery or/and bud morphogenesis. mother-bud neck in bud1 following random budding pattern characteristic of this mutant. greatly altered cdc42-1 mutant, indicating complete dependence on organised actin cytoskeleton for polarised distribution. usual deposition ring the base growing buds lacking cdc10-11 under restrictive temperature conditions, whereas septum region absent both cdc15-lyt1 cells. These results point localisation bud-neck on both septins septum integrity. Furthermore, absence Bni4p, a scaffold protein targeting chitin synthase III complex to neck, not longer found neck large-budded cells undergoing cytokinesis. Finally, properly cells deleted CHS5 , which encodes transport of Chs3p, completely mislocalised sbe2/sbe22 mutants, suggesting systems Chs3p are certain extent coincident. other GPI-cell proteins, such as Cwp1p, however, does depend these the latter either mutants.