The global transcriptional response to transient cell wall damage in Saccharomyces cerevisiae and its regulation by the cell integrity signaling pathway.

作者: Raúl García , Clara Bermejo , Cecilia Grau , Rosa Pérez , Jose Manuel Rodríguez-Peña

DOI: 10.1074/JBC.M312954200

关键词:

摘要: In the yeast Saccharomyces cerevisiae, environmental stress conditions that damage cell wall lead to activation of so-called "compensatory mechanism," aimed at preserving integrity through a remodeling this extracellular matrix. Here we used DNA microarrays investigate molecular basis response two agents induce transient damage; namely Congo Red and Zymolyase. Treatment cells with these elicited up-regulation 132 101 genes respectively, main functional groups among them being involved in construction metabolism. The does not occur until hours after exposure wall-perturbing agent. some cases, was transient, but more sustained others, especially case remodeling. Clustering data together those from constitutive damage, revealed existence cluster co-regulated strongly induced under all assayed. Those by showed an enrichment binding motifs for Rlm1p, Crz1p, SBF (Swi4p/Swi6p), Msn2p/Msn4p, Ste12p, Tec1p transcription factors, suggesting complex regulation possible involvement several signaling pathways. With exception PHO89 FKS2, none up-regulated slt2 strain. Moreover, characterization transcriptional rlm1 mutant strain only few (i.e. PHO89, YLR042C, CHA1) were least partially independently factor rest totally dependent on their activation. Our findings consistently demonstrate pathway regulates compensatory response, mainly mediated Rlm1p.

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