Endothelial Activation by Adenosine and Coronary Flow Regulation in the Guinea Pig Heart
作者： K. Kroll , J. Schrader , D. Möllmann
DOI: 10.1007/978-3-642-45619-0_39
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摘要: In order to investigate the relationship between receptor-mediated activation of coronary endothelial adenylate cyclase and changes in flow, adenine nucleotides isolated guinea pig hearts were selectively prelabeled by an intracoronary infusion 3H-adenosine at a concentration 10−8 M. Following prelabeling, release 3H-cAMP into effluent perfusate was measured used as index cyclase. Adenosine, NECA, R-PIA, S-PIA caused parallel increases conductance efflux 3H-cAMP, both effects showed rank potency characteristic adenosine A2 receptors. Using specific radioactivity cyclic AMP released during NECA stimulation represent precursor nucleotides, contribution endothelium total cardiac calculated be 14%. Procaterol, selective beta2 adrenoceptor agonist, prostaglandin E1 also vasodilation increased without augmenting left ventricular contractile function. contrast these compounds, ATP near maximal vasodilation, but remained control levels. These results demonstrate presence A2, adrenergic, receptors that stimulate addition, data suggest distinct purinergic receptor is involved vasodilation. Activation may play minor role mediating
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