Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice.

摘要: Objective The use of transgenic and knockout mice has led to a need for consistent model mouse transient focal cerebral ischemia. In great majority the published middle artery (MCA) occlusion studies, methods indicated type intraluminal suture used without indicating actual diameter after modification. We attempted determine ideal produce in MCA adult male C57BL/6 mice. Methods Suture tips were coated depth 4 mm with glue, 6-0 sutures eight different, precisely measured diameters produced. different introduced 10 into internal carotid via external (n = 40; five animals each diameter), (22-24 g) transaortically perfused saline. base brain was exposed, photographs vessels obtained before transaortic injection Evans blue dye consistency diameter. Cerebral blood flow minutes 20 reperfusion, 2,3,5-triphenyltetrazolium chloride staining performed demonstrate ischemic damage additional 110-microm 5) 180-microm 8) sutures. Results Sutures measuring 170 microm 180 consistently occluded addition, demonstrated infarction infarct volume 36.3 +/- 4.2 mm3. Conclusion Small changes tip affect model.