Enhancement of Adenosine Triphosphatase Activity of Purified Chloroplast Coupling Factor 1 in an Aqueous rganic Solvent1

摘要: The ATPase activity of purified coupling factor 1 (CF1) spinach chloroplasts [EC 3.6.1.3] was reversibly enhanced in some aqueous organic solvents, notably methanol, ethanol, and acetone. Pretreatment CF1 with 20% (v/v) methanol did not affect the subsequent activity. depended entirely on final concentration reaction mixture. In presence Km Ca2+-ATPase from ATP lowered 0.4 mM to 0.2 mM. Not only Ca2+, but also Cd2+, Mg2+, Mn2+, Zn2+ supported at rates higher than 7 mumol.mg protein-1 . min-1. Co2+, Ni2+, Pb2+ 0.5-1.0 activities by following cations, if any, were less min-1; Ba2+, Cu2+, Fe2+, Hg2+, Sn2+, Sr2+. optimum for Mg2+-ATPase about 30% (v/v). pH values 8.0 8.8, respectively. enhancing effect solvents appears be associated their relative lipophilic character as defined octanol-water partition coefficient. th trypsin-activated heat-activated inhibited