Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum

摘要: Abstract Heliobacterium modesticaldum is an anaerobic photosynthetic bacterium that grows optimally at pH 6–7 and 52 °C the only phototrophic member of Firmicutes phylum family (gram-positive bacteria with low GC content). The ATP synthase H. was isolated characterized biochemical biophysical levels. holoenzyme exhibited subunit patterns F-type synthases containing a 5-subunit hydrophilic F 1 subcomplex 3-subunit hydrophobic 0 subcomplex. hydrolysis by HF successfully detected after pretreatment different detergents in-gel ATPase activity assay, which showed highest in presence mild such as LDAO; moreover, high catalytic gel already initial incubation period 0.5 h. In contrast, extremely harsher TODC. fully functional enzyme will form basis for future structural studies.