Inhibitory mechanism on proliferation of hepatocellular carcinoma cell line by tetrandrine

摘要: AIM: To study the mechanism of tetrandrine (TTD) on proliferation and apoptosis hepatic cell carcinoma (HCC) line. METHODS: MTT colorimetry was used to evaluate influence TTD cells. Flow cytometry test reactive oxygen species (ROS) levels in Electrophoresis 1.5 % agarose gels assess DNA laddering. RESULTS: Proliferation rate HCC line stimulated by 10 20 μmol/L at 24 h, 48 72 h 90.1±1.0 %, 77.5±2.0 70.2±2.9 56.6±1.6 61.6±2.0 47.2±1.9 respectively (F =40.025, P <0.001). O2 - H2O2. generated lines incubated with 0, for 2 hours were 35 24.5 36.6 40.5 63.2 84.6 respectively. Typical ladder observed after hours.