Immunological identification of 1,25-dihydroxyvitamin D3 receptors in human promyelocytic leukemic cells (HL-60) during homologous regulation.
作者： Y Lee , M Inaba , H F DeLuca , W S Mellon
DOI: 10.1016/S0021-9258(18)80055-2
关键词:
摘要: Immunological techniques were utilized to detect 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) receptor levels and characterize physical/chemical changes in receptors human promyelocytic leukemic cells (HL-60) during continuous exposure hormone. The monoclonal antibody (IVG8C11) raised against the porcine intestinal 1,25-(OH)2D3 immunoprecipitated quantitatively nuclear extracts from HL-60 cells. highly enriched electrophoresed on sodium dodecyl sulfate-polyacrylamide gels transferred polyvinylidene difluoride membranes, which probed with 125I-labeled IVG8C11. basal treated for 2 h was detected as a single form at 53 kDa. Moreover, shown be up-regulated 12 down-regulated 48 72 presence of hormone evidenced by ratio density bands, 1.0 (2 h):4.2 (12 h):1.2 (48 h):0.9 (72 h), measured laser scanning densitometry. up- also forms had same molecular mass receptor. Therefore, data presented here strongly support hypothesis homologous regulation intact target
sci-hub.st 参考文章(39)
K B Horwitz, W L McGuire, Nuclear mechanisms of estrogen action. Effects of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing. Journal of Biological Chemistry. ,vol. 253, pp. 8185- 8191 ,(1978) , 10.1016/S0021-9258(17)34379-X
A. E. Bolton, W. M. Hunter, The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay Biochemical Journal. ,vol. 133, pp. 529- 538 ,(1973) , 10.1042/BJ1330529
Thomas J. Chaplinski, Thomas E. Bennett, José F. Caro, Alteration in Insulin Receptor Expression Accompanying Differentiation of HL-60 Leukemia Cells Cancer Research. ,vol. 46, pp. 1203- 1207 ,(1986)
R.E. Martell, R.U. Simpson, J.M. Taylor, 1,25-Dihydroxyvitamin D3 regulation of phorbol ester receptors in HL-60 leukemia cells. Journal of Biological Chemistry. ,vol. 262, pp. 5570- 5575 ,(1987) , 10.1016/S0021-9258(18)45610-4
L.C. Pan, P.A. Price, Ligand-dependent regulation of the 1,25-dihydroxyvitamin D3 receptor in rat osteosarcoma cells. Journal of Biological Chemistry. ,vol. 262, pp. 4670- 4675 ,(1987) , 10.1016/S0021-9258(18)61247-5
V K Ostrem, W F Lau, S H Lee, K Perlman, J Prahl, H K Schnoes, H F DeLuca, Induction of monocytic differentiation of HL-60 cells by 1,25-dihydroxyvitamin D analogs. Journal of Biological Chemistry. ,vol. 262, pp. 14164- 14171 ,(1987) , 10.1016/S0021-9258(18)47919-7
Peter F. Brumbaugh, Mark R. Haussler, 1α,25-Dihydroxycholecalciferol Receptors in Intestine Journal of Biological Chemistry. ,vol. 249, pp. 1251- 1257 ,(1974) , 10.1016/S0021-9258(19)42968-2
Mark R. Haussler, Carol A. Donaldson, Shigeharu Dokoh, Influence of 1,25-Dihydroxyvitamin D3 on Cultured Osteogenic Sarcoma Cells: Correlation with the 1,25-Dihydroxyvitamin D3 Receptor Cancer Research. ,vol. 44, pp. 2103- 2109 ,(1984)
H Tanaka, E Abe, C Miyaura, T Kuribayashi, K Konno, Y Nishii, T Suda, 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60). Biochemical Journal. ,vol. 204, pp. 713- 719 ,(1982) , 10.1042/BJ2040713
K.B. Horwitz, W.L. McGuire, Estrogen control of progesterone receptor in human breast cancer. Correlation with nuclear processing of estrogen receptor. Journal of Biological Chemistry. ,vol. 253, pp. 2223- 2228 ,(1978) , 10.1016/S0021-9258(17)38062-6