Comparison of colony formation in adult mouse spermatogonial stem cells developed in Sertoli and STO coculture systems
作者： S. M. Mohamadi , M. Movahedin , S. M. Koruji , M. Asghari Jafarabadi , Z. Makoolati
DOI: 10.1111/J.1439-0272.2011.01201.X
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摘要: Summary This study aimed to compare the in vitro effects of coculture with Sertoli and SIM mouse embryo-derived thioguanine- ouabain-resistant (STO) feeder layer cells on efficiency adult spermatogonial stem (SSCs) colony formation. SSCs were isolated from testes, their identity was confirmed using immunocytochemistry against Oct4, CDH1, PLZF C-kit for vimentin cells. cultured a simple culture system (control group) top STO layers 2 weeks. The number diameter colonies evaluated during third, 7th, 10th 14th day culture, expression Oct-4, α6 β1 integrins assessed quantitative RT-PCR. Significant differences observed between three groups, separately each time (P < 0.05), higher mean (P < 0.05). results RT-PCR showed gene integrin group, but no significant Oct-4 among groups. Based optimal effect Seroli formation SSCs, it is suggested use these better colonisation SSCs.
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