Effects of extracellular matrices and lectin Dolichos biflorus agglutinin on cell adhesion and self-renewal of bovine gonocytes cultured in vitro.

摘要: Surface molecules of primitive male germ cells, gonocytes, are essential components for regulating cell adhesion and maintaining self-renewal in mammalian species. In domestic animals, the stage-specific glycan epitope α-N-acetylgalactosamine (GalNAc) is recognised by lectin Dolichos biflorus agglutinin (DBA) found on surface gonocytes spermatogonia. Gonocytes from bovine testis formed mouse embryonic stem-like colonies plates that had been coated with DBA or extracellular matrix (ECM) components, such as gelatin (GN), laminin (LN) poly-L-lysine (PLL). The number DBA-coated plate was significantly higher than GN-, LN- PLL-coated plates. Pretreating to neutralise terminal GalNAc residues strongly suppressed colony formation. Furthermore, expression a cell-specific gene pluripotency-related transcription factors increased considerably These results suggest can recognise precoated resulting GalNAc–DBA complexes support stem potentials vitro. complexes, through epitope, may provide suitable microenvironment proliferation culture.