Antibiotic resistome and microbial community structure during anaerobic co-digestion of food waste, paper and cardboard.

摘要: Solid organic waste is a significant source of antibiotic resistance genes (ARGs) and effective treatment strategies are urgently required to limit the spread antimicrobial resistance. Here, we studied ARG diversity abundance as well relationship between resistome microbial community structure within lab-scale solid-state anaerobic digester treating mixture food waste, paper cardboard. A total 10 samples from feed digestion products were collected for analysis including small subunit rRNA gene sequencing, metagenome sequencing high-throughput quantitative PCR. We observed shift in composition reduction after 6 weeks digestion. ARGs identified all with multidrug being most abundant type. Thirty-two per cent detected located on plasmids indicating potential horizontal transfer. Using metagenomic assembly binning, bacterial hosts feed, which included Erwinia, Bifidobacteriaceae, Lactococcus lactis Lactobacillus. Our results indicate that process sequential cardboard tested herein provides relative 16S gene.