Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast.

摘要: Genetic analysis of protein function requires a rapid means inactivating the gene under study. Typically, this exploits temperature-sensitive mutations or promoter shut-off techniques. We report adaptation to Schizosaccharomyces pombe anchor-away technique, originally designed in budding yeast by Laemmli lab. This method relies on rapamycin-mediated interaction between FRB- and FKBP12-binding domains relocalize nuclear proteins interest cytoplasm. demonstrate depletion abundant as proof principle.