Cold active holocellulase cocktail from Aspergillus niger SH3: process optimization for production and biomass hydrolysis

摘要: Abstract The conversion of cellulosic biomass into monomeric sugars is the most costly and energy intensive process in bioethanol production. Cold active cellulases produced by psychrophilic microorganisms can counter this limitation hydrolyzing at low temperature. Enrichment soil samples collected from Himalayan region India, 5 °C molecular identification revealed prominence phylum ascomycota. hydrolytic potential all isolates terms xylanases were tested different temperatures isolate A. niger SH3 which exhibited significantly higher enzyme production Thereafter, experimental mixture design was employed to study influence carbon sources ( e.g . wheat bran, straw paddy straw). gave highest titer endoglucanase (655.94 IU/g), β -glucosidase (540.57 IU/g), FPase (31.48 IU/g) xylanase (1951.63 IU/g) under optimized conditions after 7 days solid state fermentation. Hydrolysis pretreated using crude enzymes (15 FPU/gds) 40 °C resulted maximum sugar yield (375.84 ± 0.23 mg/gds). This proves secretome for efficient hydrolysis temperature its suitability developing an biofuel process.