Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis.

摘要: Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase commercial enzyme leads to incomplete biomass hydrolysis. decomposition at high temperature environments us isolate thermotolerant microbes with production potential. A total 11 isolates were obtained from compost cow dung samples that able grow 50 °C. On basis qualitative quantitative estimation production, Bacillus subtilis RA10 was selected for further studies. medium components growth conditions optimized enhanced up 19.8-fold. B. retained 78% activity 80 °C 68.32% stable even 50 °C after 48 h incubation. supplementation into cellulase resulted 1.34-fold higher glucose release. Furthermore, also functionally elucidated by cloning overexpression full length GH1 family gene RA10. purified protein characterized as thermostable enzyme. would facilitate efficient saccharification cellulosic fermentable sugar. Consequently, saccharification, be recovered reused reduce cost overall bioethanol process.