A new twist on plasma membrane repair.

摘要: Cells in multicellular organisms are under constant mechanical stress, and often the plasma membrane (PM) is compromised. Fortunately, there a vigorous repair mechanism that rapidly (within seconds) reseals wound site by fusion with an internal patch. Downstream events, remodeling of injury site, forming replacement PM, must be carried out quickly minutes) if cell to survive multiple sequential injuries. The repertoire proteins required breaks (the PM repairome) one major unknowns this area research. As initial approach defining repairome, surface biotinylation protocol was developed identify intracellular become exposed at reversible injury. It likely least some these important mediators repair. These studies led surprising finding, namely identification nuclear endoplasmic reticulum resident transiently su...