Mannitol-specific enzyme II of the bacterial phosphotransferase system. III. The nucleotide sequence of the permease gene.
作者： C A Lee , M H Saier
DOI: 10.1016/S0021-9258(17)44522-4
关键词:
摘要: The nucleotide sequence of the mtlA gene, which codes for mannitol-specific Enzyme II Escherichia coli phosphotransferase system, is presented. From gene sequence, primary translation product predicted to consist 637 amino acids (Mr = 67,893). This result compared acid composition and molecular weight purified mannitol protein. hydrophobic hydrophilic properties enzyme were evaluated along its using a computer program (Kyte, J., Doolittle, R. F. (1982) J. Mol. Biol. 157, 105-132). analysis predicts that NH2-terminal half resides within membrane, whereas COOH-terminal has soluble possible functions such protein structure are discussed. RNA mapping identified promoter mRNA start point mtl operon.
sci-hub.st 参考文章(29)
B Erni, H Trachsel, P W Postma, J P Rosenbusch, Bacterial phosphotransferase system. Solubilization and purification of the glucose-specific enzyme II from membranes of Salmonella typhimurium. Journal of Biological Chemistry. ,vol. 257, pp. 13726- 13730 ,(1982) , 10.1016/S0021-9258(18)33508-7
G R Jacobson, C A Lee, J E Leonard, M H Saier, Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease. Journal of Biological Chemistry. ,vol. 258, pp. 10748- 10756 ,(1983) , 10.1016/S0021-9258(17)44520-0
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
Werner Kundig, Saul Roseman, Sugar Transport I. ISOLATION OF A PHOSPHOTRANSFERASE SYSTEM FROM ESCHERICHIA COLI Journal of Biological Chemistry. ,vol. 246, pp. 1393- 1406 ,(1971) , 10.1016/S0021-9258(19)76986-5
G.R. Jacobson, C.A. Lee, M.H. Saier, Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system. Journal of Biological Chemistry. ,vol. 254, pp. 249- 252 ,(1979) , 10.1016/S0021-9258(17)37905-X
J E Leonard, M H Saier, Mannitol-specific enzyme II of the bacterial phosphotransferase system. II. Reconstitution of vectorial transphosphorylation in phospholipid vesicles. Journal of Biological Chemistry. ,vol. 258, pp. 10757- 10760 ,(1983) , 10.1016/S0021-9258(17)44521-2
Byron Anderson, Nancy Weigel, Werner Kundig, Saul Roseman, Sugar Transport III. PURIFICATION AND PROPERTIES OF A PHOSPHOCARRIER PROTEIN (HPR) OF THE PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI Journal of Biological Chemistry. ,vol. 246, pp. 7023- 7033 ,(1971) , 10.1016/S0021-9258(19)45945-0
J E Leonard, M H Saier, Genetic dissection of catalytic activities of the Salmonella typhimurium mannitol enzyme II. Journal of Bacteriology. ,vol. 145, pp. 1106- 1109 ,(1981) , 10.1128/JB.145.2.1106-1109.1981
M H Saier, M R Schmidt, Vectorial and nonvectorial transphosphorylation catalyzed by enzymes II of the bacterial phosphotransferase system. Journal of Bacteriology. ,vol. 145, pp. 391- 397 ,(1981) , 10.1128/JB.145.1.391-397.1981
E. Solomon, E. C. C. Lin, Mutations Affecting the Dissimilation of Mannitol by Escherichia coli K-121 Journal of Bacteriology. ,vol. 111, pp. 566- 574 ,(1972) , 10.1128/JB.111.2.566-574.1972