Structure and evolution of a multidomain multiphosphoryl transfer protein. Nucleotide sequence of the fruB(HI) gene in Rhodobacter capsulatus and comparisons with homologous genes from other organisms.

摘要: The gene order of the fructose ( fru ) operon and nucleotide sequence first fruB(HI) Rhodobacter capsulatus are reported, analyzed compared with homologous genes from other bacteria, products identified. Included within region reported is a encoding multiphosphoryl transfer protein (MTP) phosphoenolpyruvate: sugar phosphotransferase system (PTS). MTP consists three moieties: fructose-specific enzyme III (III )-like N-terminal moiety (residues 1 to 143) followed by an FPr(HPr)-like 157 245) I-like 273 827). III-like closely resembles 143 residues -FPR fusion Salmonella typhimurium (40·6% identity throughout its length) C-terminal 145 mannitol-specific II (II mtl (37·8% length ). FPr-like domain S. FPr (42·4% identity) Escherichia coli or HPr (38·8% identity). E. I (38·9% Predicted phosphorylation sites functional units (His62 in -like moiety; Hiauthor71 HiThe His171 His457 moiety) were identified on basis comparisons proteins enteric bacteria. domains joined two flexible “linkage” regions, rich alanine, glycine proline, which show 47% each other. They also exhibit high degree linkage mannose-specific man PTS as well several bacterial, eukaryotic viral origin. At RNA level, these linker regions formed hairpin structures (90%) G+C content.57 content. Analyses -FPr revealed that between moieties this stretch 142 amino acids do not homology know proteins. This adjacent contain 110 exhibiting 59% similarity receiver consensus motif defined Kofoid Parkinson. Because has been implicated transcriptional regulation, may prove have regulatory significance. found maize pyruvate: phosphate dikinase. Three observed dikinase: (1)