Unique monocistronic operon (ptsH) in Mycoplasma capricolum encoding the phosphocarrier protein, HPr, of the phosphoenolpyruvate:sugar phosphotransferase system. Cloning, sequencing, and characterization of ptsH.

摘要: The region of the genome Mycoplasma capricolum encompassing gene (ptsH) encoding HPr, a general energy-coupling protein phosphoenolpyruvate:sugar phosphotransferase system, was cloned and sequenced. Examination sequence revealed unique arrangement ptsH gene. In all other bacterial species characterized thus far, is part polycistronic operon that includes (ptsI) Enzyme I system; M. monocistronic situated between two open reading frames unrelated to system function. immediately upstream codes for helicase, frame downstream ptsH, although not homologous any previously identified protein, contains signature characteristic [C-5] cytosine-specific DNA methylases. product has characteristics similar HPr produced by Gram-positive organisms: it greater similarity HPrs bacteria than those Gram-negative organisms, phosphorylated kinase derived from complements sugar phosphorylation activity in extracts. high calculated isoelectric point (pI = 9.18) absence glutamate residues C-terminal distinguish described HPrs.