Synthesis of a BODIPY Library and Its Application to the Development of Live Cell Glucagon Imaging Probe
作者： Jun-Seok Lee , Nam-young Kang , Yun Kyung Kim , Animesh Samanta , Suihan Feng
DOI: 10.1021/JA9011657
关键词:
摘要: The first BODIPY library (BD) was synthesized, and a highly selective glucagon sensor, Glucagon Yellow (BD-105), discovered by fluorescence image-based screening method. BD synthesized via Knoevenagel-type condensation reaction with 160 benzaldehydes the 1,3 dimethyl-BODIPY scaffold. Using compounds, performed against three cell lines including AlphaTC1 BetaTC6 cells which secret insulin, respectively, HeLa as control cells. Out of candidate probes, one compound, Yellow, exhibited staining only in selectivity toward confirmed vitro comparison its intensity change 19 biologically relevant analytes. Subsequent immunostaining experiments revealed that antibody colocalized pancreas tissue, showing high quantitative correlation analysis Pearson’s coefficient constant (Rr = ...
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