Highly sensitive electrochemiluminescence biosensors for cholesterol detection based on mesoporous magnetic core–shell microspheres

摘要: A sensitive electrochemiluminescence (ECL) biosensor for cholesterol detection based on multifunctional core–shell structured microspheres (Fe3O4@SiO2–Au@mpSiO2) is reported. This microsphere consisted of a core silica-coated magnetite nanoparticle, an active transition layer gold nanoparticles and mesoporous silica shell. Scanning electron microscopy was employed to observe the morphology nanomaterials transmission used further confirm subtle structure Fe3O4@SiO2–Au@mpSiO2. The possessed large surface area that increased enzyme loading, enhanced ECL signal. They were immobilize oxidase with high sensitivity, low limit wide linear range. range from 0.83 2.62 mM 0.28 µM (S/N = 3). Moreover, reproducibility, stability selectivity established.