Haemolytic plaque formation by mouse peritoneal cells, and the effect on it of Friend virus infection.

摘要: Peritoneal cells from non-immunized mice, when incubated in vitro with sheep red and complement a film of carboxymethyl-cellulose gum, form plaques haemolysis after latent phase 15–20 hours. Plaques are also produced by the free pleural cavity but not lymph node, spleen, thymus bone marrow cells. at room temperature, nor has been inactivated or peritoneal have heat-killed. The phenomenon is age-dependent: reach highest activity donor mice about 10 weeks old. By testing purified populations lymphocytes macrophages cell type responsible for plaque production identified as lymphocyte. Plaque formation suppressed presence an anti-mouse immunoglobulin serum without any detectable effect on viability anticomplementary action. This suppressive destroyed prior precipitation antiserum normal mouse serum. A technique which facilitates study individual developed applied to Friend virus-infected mice. days intravenous intraperitoneal infection similar that their uninfected counterparts. killed 17 19 significantly reduced numbers plaques. accompanied decrease percentage present population infected