Functional study of a salt-inducible TaSR gene in Triticum aestivum
作者： Xiao-Li Ma , Wei-Na Cui , Qian Zhao , Jing Zhao , Xiao-Na Hou
DOI: 10.1111/PPL.12337
关键词:
摘要: The gene expression chip of a salt-tolerant wheat mutant under salt stress was used to clone salt-induced with unknown functions. This designated as TaSR (Triticum aestivum salt-response gene) and submitted GenBank accession number EF580107. Quantitative polymerase chain reaction (PCR) analysis showed that induced by stress. Arabidopsis rice (Oryza sativa) plants expressing presented higher tolerance than the controls, whereas AtSR RNA interference were more sensitive salt. Under stress, reduced Na(+) concentration improved cellular K(+) Ca(2+) concentrations; this also localized on cell membrane. β-Glucuronidase (GUS) staining GUS fluorescence quantitative determination conducted through fragmentation cloning promoter. Salt stress-responsive elements detected at 588-1074 bp upstream start codon. tests full-length promoter in different tissues indicated activity highest leaf Bimolecular complementation yeast two-hybrid screening further correlation TaPRK TaKPP. In vitro phosphorylation TaPRK2697 did not phosphorylate TaSR. study revealed novel may be improve plant
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