Quantification of zolpidem in human plasma by high-performance liquid chromatography with fluorescence detection.

摘要: A simple, reliable HPLC method with fluorescence detection (excitation 320 and emission 388 nm) was developed validated for quantitation of zolpidem in human plasma. Following a single-step liquid-liquid extraction, the analyte internal standard (quinine) were separated using an isocratic mobile phase on reversed-phase C(18) column. The lower limit 1.8 ng/mL, relative deviation less than 5%. linear dynamic range 1.8-288 ng/mL established. This between-batch within-batch precision 1.7-4.8 1.2-2.3%, respectively. accuracy 95.3-100.4 95.5-102.7%, Frequently coadministered drugs did not interfere described methodology. Stability plasma excellent, no evidence degradation during sample processing (autosampler) 30 days storage freezer. is simple repeatable enough to be used pharmacokinetic studies.