Detection of Ehrlichia risticii, the agent of Potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.) from northern California.

作者: Jeffrey E. Barlough , Gerhard H. Reubel , John E. Madigan , Larisa K. Vredevoe , Paul E. Miller

DOI: 10.1128/AEM.64.8.2888-2893.1998

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摘要: Ehrlichia DNA was identified by nested PCR in operculate snails (Pleuroceridae: Juga spp.) collected from stream water a northern California pasture which Potomac horse fever (PHF) is enzootic. Sequencing of PCR-amplified suite genes (the 16S rRNA, groESL heat shock operon, 51-kDa major antigen genes) indicated that the source organism closely resembled risticii, causative agent PHF. The minimum percentage spp. harboring population studied 3.5% (2 57 snails). No ehrlichia found tissues 123 lymnaeid, physid, and planorbid at same site. These data suggest pleurocerid may play role life cycle E. risticii California.

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