Identification of Full-Length Dentin Matrix Protein 1 in Dentin and Bone

摘要: Dentin matrix protein 1 (DMP1) has been identified in the extracellular (ECM) of dentin and bone as processed NH2-terminal COOH-terminal fragment. However, full-length form DMP1 not these tissues. The focus this investigation was to search for intact bone. We used two types anti-DMP1 antibodies identify DMP1: one type specifically recognizes region other is only reactive amino acid sequence. An ∼105-kDa protein, extracted from ECM rat bone, recognized by both antibodies; migration rate identical recombinant mouse made eukaryotic cells. concluded that DMP1, which considerably less abundant than its fragments also detected extract dental pulp/odontoblast complex dissected teeth. In addition, immunofluorescence analysis showed MC3T3-E1 cells are distributed differently. Our findings indicate majority must be cleaved within synthesize it minor amounts uncleaved molecules secreted into