Identification ofCandidaspecies using PCR-RFLP in cancer patients in Iran

摘要: Opportunistic infections caused by Non-Candida albicans. have been increasing. Traditional methods that are used to identify clinical isolates of Candida species time-consuming and not appropriate for rapid, accurate reliable identification. Purpose: To spp isolated from cancer patients using PCR-restriction enzyme. Materials ethods: Using universal primers, ITS1 ITS4, in this study, we could amplify ITS1-5.8S-ITS2 rDNA regions at both 80 3 standard strains. The PCR products were digested with two restriction enzymes MspI BlnI separately. Result: We successfully identified all (MspI, BlnI). albicans was the most common (77.5%), followed C. glabrata (15%), tropicalis (5%), krusei (2.5%). Although primers enzyme had ability parapsilosis, guilliermondii, dubliniensis, present did include these among ones. Conclusion: RFLP-PCR ITSI ITS4 is a easy, also applicable method laboratory identification medically important spp.