miRNA Profiling of Naïve, Effector and Memory CD8 T Cells

摘要: microRNAs have recently emerged as master regulators of gene expression during development and cell differentiation. Although profound changes in also occur antigen-induced T differentiation, the role miRNAs process is not known. We compared miRNA profiles between antigen-specific naive, effector memory CD8+ cells using 3 different methods-small RNA cloning, microarray analysis real-time PCR. many were expressed all subsets, frequency 7 (miR-16, miR-21, miR-142-3p, miR-142-5p, miR-150, miR-15b let-7f) alone accounted for ∼60% miRNAs, their was several fold higher than other miRNAs. Global downregulation (including 6/7 dominantly miRNAs) observed to naive levels tended come back up cells. However, a few notably miR-21 These results suggest that concomitant with expression, profile dynamically Sequence cloned mature revealed an extensive degree end polymorphism. While 3′end polymorphisms dominated, heterogeneity at both ends, resembling drosha/dicer processing shift seen miR-142, suggesting possible novel mechanism generate new and/or diversify target selection. Overall, our dynamic may be important regulation Our study suggests mechanisms biogenesis function.