Epression of the Apoptosis-Inducing FAS Ligand (FASL) in Human First and Third Trimester Placenta and Choriocarcinoma Cells

摘要: The Fas (Apo-1/CD95) ligand (FasL) belongs to the tumor necrosis factor family and acts through its receptor (FasR/Apo-1/CD95) induce apoptosis in target cells. FasL is expressed several immunologically privileged sites. Induction of by invading lymphocytes as a mechanism immune privilege important preventing graft rejection. Furthermore, expresssed certain malignancies it has been implicated possible key these tumors. Since placental trophoblast another very site with status, we investigated whether normal tumoral human placenta. For this purpose, mRNA was extracted from first third trimester samples well JEG3 choriocarcinoma cells reverse transcribed obtain cDNAs. These were used templates for PCR analysis expression, which specific primers employed amplify an 853 bp fragment s...