Evaluation of the immunogenicity of a recombinant HSV-1 vector expressing human group C rotavirus VP6 protein.
作者： Rosana P. Rota , Carlos A. Palacios , C. Facundo Temprana , Marcelo H. Argüelles , Marcelo G. Mandile
DOI: 10.1016/J.JVIROMET.2018.02.020
关键词:
摘要: Group C Rotavirus (RVC) has been associated globally with sporadic outbreaks of gastroenteritis in children and adults. RVC also infects animals, interspecies transmission reported as well its zoonotic potential. Considering genetic diversity the absence effective vaccines, it is important necessary to develop new generation vaccines against for both humans animals. The aim present study was characterize an HSV-1-based amplicon vector expressing a human RVC-VP6 protein evaluate humoral immune response induced after immunizing BALB/c mice. Local fecal samples positive were used isolation sequencing vp6 gene, which phylogenetically belongs I2 genotype. We show here that cells infected HSV[VP6C] efficiently express VP6 protein, specific anti-RVC antibodies mice immunized HSV[VP6C], prime-boost schedule. This work highlights vectors are attractive platform safe immunogens RVC, without addition external adjuvants.
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