Sugar transporters in the black truffle Tuber melanosporum: from gene prediction to functional characterization.
作者： Paola Ceccaroli , Roberta Saltarelli , Emanuela Polidori , Elena Barbieri , Michele Guescini
DOI: 10.1016/J.FGB.2015.05.006
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摘要: Abstract In a natural forest ecosystem, ectomycorrhiza formation is way for soil fungi to obtain carbohydrates from their host plants. However, our knowledge of sugar transporters in ectomycorrhizal ascomycetous limited. To bridge this gap we used data obtained the sequenced genome fungus Tuber melanosporum Vittad. search transporters. Twenty-three potential hexose were found, and three them (Tmelhxt1, Tmel2281 Tmel131), differentially expressed during life cycle, investigated. The heterologous expression Tmelhxt1 an hxt-null Saccharomyces cerevisiae strain restores growth glucose fructose. functional characterization profiles symbiotic phase suggest that they are high affinity at plant–fungus interface. On contrary, Tmel131 preferentially fruiting body its inability restore S. mutant led us hypothesize it could be involved transport alternative carbon sources important hypothetical saprophytic strategy complete maturation carpophore.
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