A quantitative analysis of proflavine binding to polyadenylic acid, polyuridylic acid, and transfer RNA.

摘要: Absorbance measurements show that the binding of proflavine to poly (A) and poly(U) can be quantitatively resolved (within ± 10% relative error) by consideration two bound species whose spectral properties mechanisms interaction are similar those found in DNA. proflavin systems: cooperative along phosphate backbone (process I) between aromatic rings base dye resulting presumably from some kind interacalation ligand consecutive bases II). The comparison with a simple theoretical model shows process I II not independent but coupled. The spectrophotometric method is able resolve tRNA same accuracy as obtained for poly(A) poly(U). Accordingly, it proposed mode all classes polynucleotides (DNA, RNA, synthetic polymers) nature. From differences observed distribution complexes, existence close correlation secondary structure polymer its ability complex proposed.