Sequence and structure of a serine transfer RNA with GCU anticodon from mosquito mitochondria

作者: Donald T. Dubin , Chuen-Chin Hsuehen , Graham R. Cleaves , Kathleen D. Timko

DOI: 10.1016/0022-2836(84)90423-6

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摘要: Abstract We have determined the primary sequence and modification status of a transfer RNA from mosquito mitochondria whose GCU anticodon indicates that it is serine tRNA (tRNAGCUSer), obtained information on higher order structure using partial digestion with nucleases S1 T1 under non-denaturing conditions. Although its homology to mammalian mitochondrial tRNAGCUSer modest (46%), resembles counterpart in plausible secondary configuration includes drastically abbreviated D arm six base-pair stem. Other unusual features include ribose-methylated cytidine residue at end stem, likely occurrence Ψ between amino acid IV.

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