In vitro bone engineering based on polycaprolactone and polycaprolactone–tricalcium phosphate composites

摘要: The filling of bone defects in load-bearing areas requires scaffolds possessing physical properties that are the range those host bone. In this report, composite comprising medical-grade polycaprolactone and tricalcium phosphate (mPCL-TCP) (80:20), which have been designed for applications, characterized compared with mPCL scaffolds, using vitro studies. exhibited improved hydrophilicity, compressive modulus strength. Human alveolar osteoblasts (AOs) grown on achieved higher seeding efficiencies more uniform distribution when preparations alone. AOs demonstrated better proliferation, denser multilayered cell-sheets showed earlier expression matrix-related proteins than during 28 days culture. calcium content media decreased both scaffold/cell constructs. Alkaline phosphatase activity increased significantly matrices after osteogenic induction but no distinct change was observed composite. Osteocalcin down-regulated by up-regulated at RNA protein level. Immuno-reactive signals osteopontin collagen type I, combination mineral nodules were found to be stronger mPCL-TCP scaffolds. We conclude hydrophilic had mechanical over Moreover, primary cell different matrix patterns Copyright © 2006 Society Chemical Industry