Retrospective assessment of radiation exposure using biological dosimetry: chromosome painting, electron paramagnetic resonance and the glycophorin a mutation assay.

摘要: Biological monitoring of dose can contribute important, independent estimates cumulative radiation exposure in epidemiological studies, especially studies which the physical dosimetry is lacking. Three biodosimeters that have been used to estimate past from external sources will be highlighted: chromosome painting or FISH (fluorescence situ hybridization), glycophorin A somatic mutation assay (GPA), and electron paramagnetic resonance (EPR) with teeth. All three applied A-bomb survivors, Chernobyl clean-up workers, workers. Each biodosimeter has unique advantages limitations depending upon level type exposure. Chromosome most widely exposure, results these provide evidence dose-related translocations persist for decades. EPR tooth validate models acute chronic although present requirement extracted teeth a disadvantage. GPA correlated physically based after high-dose, exposures but not low-dose, exposures. Interindividual variability appears limitation both GPA. Both techniques population, whereas individual This paper review each compare their application selected studies.