Capillary Electrophoretic Separation of Nuclei Released from Single Cells

摘要: We report here the first capillary electrophoresis analysis of intact nuclei released on-column from single cells. Expression nuclear-targeted protein nuDsRed2 and plasma membrane-bound farnesylated enhanced green fluorescent in cultured human ΔH2-1 cells allowed monitoring fate these subcellular compartments upon injection a cell into separation capillary. On-column treatment with digitonin for membrane nucleus as indicated by their selective laser-induced fluorescence detection two separate spectral regions. The data suggest that less than 0.1% remains bound to individually detected nuclei. In digitonin-treated cells, electropherograms consisted prominent peak attributed localized collection weakly events (barely distinguishable scattering) seem indicate additional localization this o...