SNP Detection from De Novo Transcriptome Sequencing in the Bivalve Macoma balthica: Marker Development for Evolutionary Studies

摘要: Hybrid zones are noteworthy systems for the study of environmental adaptation to fast-changing environments, as they constitute reservoirs polymorphism and key maintenance biodiversity. They can move in relation climate fluctuations, temperature affect both selection migration, or remain trapped by physical barriers. There is therefore a very strong incentive dynamics hybrid subjected variations. The infaunal bivalve Macoma balthica emerges model species, divergent lineages hybridize, its native NE Atlantic range currently contracting North. To investigate functioning M. balthica, we developed new molecular markers sequencing collective transcriptome 30 individuals. Ten individuals were pooled each three populations sampled at margins two zones. A single 454 run generated 277 Mb from which 17K SNPs detected. SNP density averaged 1 polymorphic site every 14 19 bases, mitochondrial nuclear loci, respectively. An external file that holds picture, illustration, etc. Object name pone.0052302.e001.jpg scan detected high genetic divergence among several hundred SNPs, some them involved energetic metabolism, cellular respiration physiological stress. population differentiation, recorded nuclear-encoded ATP synthase NADH dehydrogenase well most suggests cytonuclear incompatibilities. Results this will help pave way high-resolution zone relative importance endogenous exogenous barriers gene flow system.