作者: Alan J. Kingsman , Bernard P. Mahon , Kingston H.G. Mills , Susan M. Kingsman , Amanda L. Barnard
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摘要: We have investigated Th cell recognition of the HIV core protein p24 by using CD4+ T clones derived from cynomolgus macaques immunized with hybrid p24: Ty virus-like particles (VLP). lines two animals responded to Ty-VLP, control purified p24, and whole inactivated HIV, indicating presence cells specific for as well carrier protein. The determinants recognized were identified a series overlapping peptides synthesized according sequence p24. Both peptide 11 (amino acids 235-249) 14 265-279). In addition, one line also 9 215-229). Definitive identification epitopes on was confirmed at clonal level: total four generated lines, respond specifically 14. MHC class II-restricted secreted IL-2 in response stimulation or single synthetic peptide. specificity variant demonstrated cross-reactivity simian immunodeficiency virus isolates, but only limited responses HIV-2 sequences. However, are highly conserved between 12 HIV-1 isolates both primates. These sequences may therefore be useful priming broadly reactive immune HIV-1.