Baseline microbiota activity and initial bifidobacteria counts influence responses to prebiotic dosing in healthy subjects

摘要: Summary Background  Dietary intervention with prebiotics can cause changes in the colonic microbiota and their metabolic activities. Aim  To investigate whether response to prebiotic dosing is influenced by baseline activity of flora bifidobacteria counts. Methods  The 4-week effect lactulose (10 g bid.; n = 29) oligofructose-enriched inulin n = 19) was evaluated healthy human volunteers. Lactose-[15N, 15N]-ureide used study NH3-metabolism. Urine (48 h) faeces (72 h) were collected analysed for p-cresol 15N-content gas chromatography–mass spectrometry isotope ratio mass spectrometer, respectively. Faecal quantified real-time polymerase chain reaction. Results  After administration period, urinary excretion 15N significantly decreased both groups (P < 0.05) corresponding a higher faecal (P < 0.05). decrease correlated levels (P < 0.05), indicating that subjects showed dosing. Furthermore, significant correlation seen between counts intake (P < 0.05). Conclusion  dosing, as indicated fate NH3, bifidobacteria, determined initial conditions.