Sevoflurane Postconditioning Reduces Hypoxia-Reoxygenation Injury in H9C2 Embryonic Rat Cardiomyocytes and Targets the STRADA Gene by Upregulating microRNA-107

摘要: BACKGROUND Sevoflurane as a widely used inhalational general anesthetic that also has cardioprotective role in hypoxia-reoxygenation (H/R) injury. This study aimed to investigate the effects of microRNA-107 (miR-107) on sevoflurane postconditioning (SpostC) H9C2 embryonic rat cardiomyocytes and use bioinformatics analysis identify molecular basis cardioprotection from human cardiac tissue. MATERIAL AND METHODS The STRADA gene was identified Gene Expression Omnibus (GEO) database. were cultured with sevoflurane. Quantitative real-time polymerase chain reaction (qRT-PCR) Western blot measure mRNA expression protein miR-107 cells. TargetScanHuman version 7.2 target predict 3'-UTR binding site miR-107. dual-luciferase reporter assay measured relative luciferase activity. cell proliferation rate apoptosis using MTT flow cytometry, respectively. RESULTS H/R injury cells following SpostC resulted increased reduced STRADA. Specific 3'-UTR. Upregulation injured promoted proliferation, apoptosis, downregulating caspase-3. overexpression mimic SpostC. CONCLUSIONS by targeting upregulating microRNA-107.