Identification of toxic nucleotide sequences

摘要: Toxic nucleic acid sequences and methods for identifying, using, screening libraries them, including in silico screening, are provided. Compositions of the invention comprise unimolecular double stranded polynucleotides comprising at least one toxicity region. include A/G UUU A/G/U, G/C AAA G/C, and/or GCCA, NUUU, wherein N is any nucleotide, or complements thereof. The also provides a method inducing toxic response cell, introducing into cell polynucleotide region sequence selected from group consisting GAAT, complement foregoing, said 5 base pairs long comprises sense antisense that substantially complementary.