Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City.

摘要: Beta-lactam resistant bacteria, which are commonly resident in tertiary hospitals, have emerged as a worldwide health problem because of ready-to-eat vegetable intake. We aimed to characterize the genes that provide resistance beta-lactam antibiotics Enterobacteriaceae, isolated from five commercial salad brands for human consumption Mexico City. In total, twenty-five samples were collected, grown blood agar plates, and bacteria biochemistry identified antimicrobial susceptibility testing was done. The carried family by endpoint PCR specific confirmed with whole genome sequencing (WGS) Next Generation Sequencing (NGS). Twelve positive cultures their microbiological distribution follows: 8.3% Enterobacter aerogene (n = 1), Serratia fonticola 16.7% marcesens 2), Klebsiella pneumoniae 50% 6) cloacae. results showed 11 colonies blaBIL (91.7%), blaSHV blaCTX (97.7%), 12 blaDHA (100%), four blaVIM (33.3%), two blaOXA (16.7%), blaIMP one blaKPC (8.3%), blaTEM (8.3%) gen; all negative blaROB, blaCMY, blaP, blaCFX blaLAP gene. analysis revealed genotype cloacae (blaSHV-12, blaCTX-M-15, blaDHA-1, blaKPC-2); marcescens (blaSHV-1, blaCTX-M-3, blaVIM-2); blaDHA-1); blaVIM-1, and, blaCTX-M-1, blaVIM-2, blaOXA-9). Our indicate beta-lactam-resistant acquired integrons different number pan-resistance antibiotics, including penicillins, oxacillins, cefalosporins, monobactams, carbapenems, imipenems.