Immobilization of message RNA directly from cells onto filter material

摘要: Message RNA is immobilized directly from cells onto filter material. Immobilization carried out by solubilizing cellular components with a chaotropic salt, passing the resultant solubilized through which selectively binds message and baking containing bound RNA. The salt preferably sodium iodide, potassium iodide or perchlorate. Prior to solubilizing, may be washed lysed. can hybridized labeled probe amount of measured. baking, incubated in solution acelylates basic protein other molecules might interfere molecular hybridization.