Monoclonal antibodies against O and K antigens of uropathogenic Escherichia coli O4 : K12 : H− as opsonins
作者： Chiyoji Abe , Sylvia Schmitz , Barbara Jann , Klaus Jann
DOI: 10.1111/J.1574-6968.1988.TB02988.X
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摘要: Monoclonal antibodies of subclasses IgG1 and IgG2b specific for the O4 antigen Escherichia coli 20025 (O4 : K12 H−) capsular polysaccharide same strain (IgM) were obtained with hybridoma technique using spleen cells from Balb/c mice, immunized a crude bacterial extract, Sp2/O-Ag8 myeloma cells. The anti-O4 reacted exclusively lipopolysaccharide not those serologically O-cross reactive E. coli. anti-K12 recognized as epitope (part of) KDO moiety polysaccharide. Not only anti-K12, but also effectively phagoopsonized encapsulated 20025. opsonized bacteria killed in subsequent vitro phagocytosis by human leokocytes presence serum complement.
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