Molecular cloning of F11 fimbriae from a uropathogenic Escherichia coli and characterization of fimbriae with polyclonal and monoclonal antibodies
作者： Johannes M. Ree , Paul Schwillens , Johannes F. Bosch
DOI: 10.1111/J.1574-6968.1985.TB00841.X
关键词:
摘要: The genes coding for F11 fimbriae from the uropathogenic Escherichia coli C1976 were cloned by a cosmid cloning procedure. Two clones expressed and these possessed an identical DNA fragment of 8.9 kb. This was subcloned into pBR322 this plasmid still produced caused mannose-resistant haemagglutination (MRHA). Polyclonal monoclonal antibodies against purified fimbriae. Both types used in inhibition tests MRHA adherence bacteria to uroepithelial cell line T24. After preincubation with polyclonal antiserum MR totally inhibited. Preincubation did not inhibit adherence.
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sci-hub.se 参考文章(25)
M. Norgren, S. Normark, D. Lark, P. O'Hanley, G. Schoolnik, S. Falkow, C. Svanborg-Edén, M. Båga, B.E. Uhlin, Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation. The EMBO Journal. ,vol. 3, pp. 1159- 1165 ,(1984) , 10.1002/J.1460-2075.1984.TB01945.X
F.P. Lindberg, B. Lund, S. Normark, Genes of pyelonephritogenic E. coli required for digalactoside-specific agglutination of human cells. The EMBO Journal. ,vol. 3, pp. 1167- 1173 ,(1984) , 10.1002/J.1460-2075.1984.TB01946.X
Lars Åke Hanson, Paul Kallós, Otto Westphal, Host parasite relationships in Gram-negative infections. Basel, Switzerland; S. Karger AG. ,vol. 33, ,(1983)
C. Svanborg Edén, L. Hagberg, L. A. Hanson, T. Korhonen, H. Leffler, S. Olling, Adhesion ofEscherichia coliin Urinary Tract Infection Ciba Foundation Symposium 80 - Adhesion and Microorganism Pathogenicity. ,vol. 80, pp. 161- 187 ,(1981) , 10.1002/9780470720639.CH11
Ida Ørskov, Frits Ørskov, Serology of Escherichia coli Fimbriae Progress in allergy. ,vol. 33, pp. 80- 105 ,(1983) , 10.1159/000318324
H. Schulte-Wissermann, Clinical, bacteriological and immunological aspects of urinary tract infections in children. Georg Thieme Verlag. ,(1982)
R A Hull, R E Gill, P Hsu, B H Minshew, S Falkow, Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infection and Immunity. ,vol. 33, pp. 933- 938 ,(1981) , 10.1128/IAI.33.3.933-938.1981
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
New method for isolation of immunologically pure pili from Escherichia coli Infection and Immunity. ,vol. 27, pp. 569- 575 ,(1980) , 10.1128/IAI.27.2.569-575.1980
Mannose-sensitive and mannose-resistant adherence to human uroepithelial cells and urinary virulence of Escherichia coli. Infection and Immunity. ,vol. 29, pp. 226- 233 ,(1980) , 10.1128/IAI.29.1.226-233.1980