4-O-Methylhonokiol Influences Normal Cardiovascular Development in Medaka Embryo.

摘要: Although 4-O-Methylhonokiol (MH) effects on neuronal and immune cells have been established, it is still unclear whether MH can cause a change in the structure function of cardiovascular system. The overarching goal this study was to evaluate MH, isolated from Magnolia grandiflora, development heart vasculature Japanese medaka model vivo predict human health risks. We analyzed toxicity different life-stages embryos. uptake into embryos quantified. LC50 two exposure windows (stages 9⁻36 (0⁻6 days post fertilization (dpf)) 25⁻36 (2⁻6 dpf)) were 5.3 ± 0.1 μM 9.9 0.2 μM. Survival, deformities, hatch, larval locomotor response Wnt 1 overexpressed MH-treated indicating deregulation signaling pathway, which associated with spinal cardiac ventricle deformities. Overexpression major proinflammatory mediators biomarkers detected. Our results indicated that differential sensitivity developmental stage-specific. Furthermore, demonstrated certain molecules serve as promising markers at transcriptional phenotypical levels, responding absorption developing embryo.