Inulinase Immobilized Lectin Affinity Magnetic Nanoparticles for Inulin Hydrolysis
作者： Ulviye Kilimci , Sinem Evli , Baha Öndeş , Murat Uygun , Deniz Aktaş Uygun
DOI: 10.1007/S12010-020-03476-7
关键词:
摘要: In this presented paper, concanavalin A–modified cysteine-functionalized Fe3O4/Ag core/shell magnetic nanoparticles were synthesized and used as a support material for inulinase enzyme, which has been intensively the preparation of high-fructose syrup by hydrolyzing inulin. Inulinase adsorption capacity Con A–functionalized Ag-coated was optimized changing medium pH, temperature, initial concentration, maximum found to be 655.32 mg/g nanoparticle using 1.00 mg/mL solution in pH 3.0 buffer system at 25 °C. Finally, efficient inulin degradation immobilized demonstrated TLC studies released fructose amount determined 0.533 only within 5 min hydrolysis. This newly developed hydrolysis strategy holds considerable promise produce many industries.
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Jan Fiedurek, Marcin Skowronek, Purification and Properties of Extracellular Endoinulinase from Aspergillus niger 20 OSM Food Technology and Biotechnology. ,vol. 44, pp. 53- 58 ,(2006)
James B. Sumner, Stacey F. Howell, THE ISOLATION OF A FOURTH CRYSTALLIZABLE JACK BEAN GLOBULIN THROUGH THE DIGESTION OF CANAVALIN WITH TRYPSIN Journal of Biological Chemistry. ,vol. 113, pp. 607- 610 ,(1936) , 10.1016/S0021-9258(18)74832-1
Prabhjot Kaur Gill, Prabhjeet Singh, Proizvodnja inulinaze: najnovija dostignuca Food Technology and Biotechnology. ,vol. 44, pp. 151- 162 ,(2006)
David S Hage, Affinity Chromatography: A Review of Clinical Applications Clinical Chemistry. ,vol. 45, pp. 593- 615 ,(1999) , 10.1093/CLINCHEM/45.5.593
Tais Garlet, Caroline Weber, Rodrigo Klaic, Edson Foletto, Sergio Jahn, Marcio Mazutti, Raquel Kuhn, Carbon Nanotubes as Supports for Inulinase Immobilization Molecules. ,vol. 19, pp. 14615- 14624 ,(2014) , 10.3390/MOLECULES190914615
Rocktotpal Konwarh, Niranjan Karak, Sudhir Kumar Rai, Ashis Kumar Mukherjee, Polymer-assisted iron oxide magnetic nanoparticle immobilized keratinase. Nanotechnology. ,vol. 20, pp. 225107- ,(2009) , 10.1088/0957-4484/20/22/225107
Ronald L. Crawford, Andrzej J. Paszczynski, Andrew K. Johnson, Anna M. Zawadzka, Lee A. Deobald, Novel method for immobilization of enzymes to magnetic nanoparticles Journal of Nanoparticle Research. ,vol. 10, pp. 1009- 1025 ,(2008) , 10.1007/S11051-007-9332-5
Marceli Fernandes Silva, Diane Rigo, Vinícius Mossi, Rogério Marcos Dallago, Pedro Henrick, Graciele de Oliveira Kuhn, Clarissa Dalla Rosa, Débora Oliveira, J. Vladimir Oliveira, Helen Treichel, Evaluation of enzymatic activity of commercial inulinase from Aspergillus niger immobilized in polyurethane foam Food and Bioproducts Processing. ,vol. 91, pp. 54- 59 ,(2013) , 10.1016/J.FBP.2012.08.003
Lata Sheo Bachan Upadhyay, Nishant Verma, Synthesis and characterization of cysteine functionalized silver nanoparticles for biomolecule immobilization Bioprocess and Biosystems Engineering. ,vol. 37, pp. 2139- 2148 ,(2014) , 10.1007/S00449-014-1191-8
Lata Sheo Bachan Upadhyay, Nishant Verma, Dual immobilization of biomolecule on the glass surface using cysteine as a bifunctional linker Process Biochemistry. ,vol. 49, pp. 1139- 1143 ,(2014) , 10.1016/J.PROCBIO.2014.04.003