Proteomic characterization of the released/secreted proteins of Leishmania (Viannia) braziliensis promastigotes.

摘要: Extracellular proteins secreted/released by protozoan parasites are key mediators of the host-parasite interaction. To characterise profile Leishmania (Viannia) braziliensis promastigotes, a proteomic approach combining two-dimensional electrophoresis (2DE), tandem matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF/TOF) mass spectrometry, and data mining was carried out. The 2DE map revealed set 270 secreted protein spots from which 42 were confidently identified classified into 11 categories according to Gene Ontology (GeneDB database) KEEG annotation biological processes. Parasite promastigotes able secrete/release involved in immunomodulation, signal transduction, intracellular survival, such as HSP70, acid phosphatase, activated kinase C receptor (LACK), elongation factor 1beta, tryparedoxin peroxidase. Data showed that approximately 5% present classical secretion whereas 57% following non-classical mechanisms, indicating export this primitive eukaryote might proceed mainly unconventional pathways. This study reports suitable identify culture supernatant L. provides new perspectives for molecules potentially early stages infection.