The effect of assay type and sample matrix on detected cytokine concentrations in human blood serum and nasal lavage fluid.

摘要: Cytokine concentrations in biological fluids are widely used markers for activation of immunological processes. Confirming the reproducibility measurements is important, especially longitudinal or multicenter studies where time between analyses different analyzing laboratories increases intra-assay variability. In this study, cytokine analysis conducted with assay platforms was studied by comparing results two cytokines [interleukin (IL)-6 serum and nasal lavage fluid (NAL) IL-8 NAL] analyzed Meso Scale Discovery (MSD) ultra-sensitive single multiplex kits (n=76). addition, difference levels sample matrices altogether 9 [IL-6, IL-2, IL-8, IL12p70, IL-1β, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)γ, IL-10 tumor necrosis (TNF)α] measured from NAL same study subjects (n=460). The show that assays concordant but not equal. Comparison revealed do correspond detected fluid. It can be concluded comparability high, should compared directly each other. differences indicate specific matrix represent distinct conditions.