Analysis of DNA damage and repair in murine leukemia L1210 cells using a quantitative polymerase chain reaction assay

摘要: Abstract The polymerase chain reaction (PCR) represents an alternative to the current methods for investigating DNA damage and repair in specific genomic segments. In theory, any lesion which blocks Taq can be measured by this assay. We used quantitative PCR (QPCR) determine frequencies produced cisplatin ultraviolet light (UV) a 2.3 kilobase (kb) segment of mitochondrial 2.6 kb DHFR gene mouse leukemia L1210 cells. The frequency UV-induced lesions increased linearly with dose, was 0.58 lesions/10 kb/10 J/m2 DNA, 0.37 gene. With cisplatin, also 0.17 microM gene, 0.07 DNA. This result is contrary that Murata et al., 1990 (1), received greater than did nuclear Using measure segment, we observed less 10% were removed 4 h, but over 70% 8 h. Repair 43% during 24 h period.